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3,3',5,5'-Tetramethylbenzidine (TMB) membrane peroxidase substrate Plus, liquid, Biotechnology Grade 1 component

Supplier: VWR Chemicals

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K830-100MLEA 7457 INR InStock
K830-100ML
3,3',5,5'-Tetramethylbenzidine (TMB) membrane peroxidase substrate Plus, liquid, Biotechnology Grade 1 component
3,3',5,5'-Tetramethylbenzidine (TMB)
Ready to use sensitive substrate for the detection of horseradish peroxidase (HRP) activity suitable for ELISA and solution assays. Offers faster signal, enhanced sensitivity and the lowest possible background.

  • Ready to use solution

Reaction of the substrate with HRP generates a free-radical cation that exhibits blue colour with an absorption maximum at 653 nm. Acidification of the reaction by addition of sulfuric acid yields a yellow-coloured diimine terminal oxidation product, which absorbs light at 450 nm.

TMB Plus liquid component substrate is optimised with TMB and hydrogen peroxide concentrations that allow a linear response to typical HRP concentrations utilised in immunologic assays.

Ordering information: Contains 2,08 mmol/l TMB, hydrogen peroxide, citric acid buffer pH 3,3 and additional proprietary, non toxic stabilisers.

Packaging: Packed in plastic bottle.


Formula: C₁₆H₂₀N₂
MW: 240,35 g/mol
Boiling Pt: 402 °C (1013 hPa)
Melting Pt: 166 to 170 °C
Storage Temperature: ambient
 MDL Number: MFCD00007748
CAS Number: 54827-17-7
EINECS: 259-364-6
UN: 0000
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Specification Test Results
Abs.@450nm (50%, 1M H2SO4) ≤ 0.1
Abs.@650nm (undiluted) ≤ 0.1
Assay (P/F) PASS / FAIL
pH @25C (undiluted) 2.6 - 3.6

 

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