L0190-500
L0191-500
L0192-500
P0191-N1L
P0191-N10L
P0191-N50L
P0192-N1L
P0192-N10L
L0190-500EA
0
INR
InStock
L0190-500
L0191-500
L0192-500
P0191-N1L
P0191-N10L
P0191-N50L
P0192-N1L
P0192-N10L
Cell culture media, Iscove's Modified Dulbecco's Medium (IMDM)
Cell Culture Media
In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.