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Betaine enhancer solution 5 M (5X) for PCR

Supplier: VWR Chemicals

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733-1361EA 0 INR InStock
733-1361 733-2450 733-2451
Betaine enhancer solution 5 M (5X) for PCR
Betaine
Betaine enhancer is especially effective when used with high GC-rich regions or templates with a high degree of secondary structures. It has a decreasing effect on the primer melting temperature.

  • Enhances amplification on GC-rich and difficult DNA targets
  • Lowers the melting temperature

The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures.

Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction.

Caution: For Research Use Only. Not for use in diagnostic procedures.


Formula: C₅H₁₁NO₂
MW: 117.15 g/mol
Storage Temperature: Freezer
 MDL Number: MFCD00012123
CAS Number: 107-43-7
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Specification Test Results
Functional test - 1x Betaine Enchancer Solution is tested for amplification of 613bp fragment from human genomic DNA using Taq DNA polymerase at standard PCR Conditions Passes test
Volume check- fill volume >= 0.1ml per tube Passes test

 

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