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DNA fragment and library prep kit, sparQ

Supplier: QIAGEN BEVERLY
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The streamlined workflow utilizes a proprietary enzyme mix that integrates tunable and reproducible fragmentation with DNA polishing simplifying library construction.
Coverage uniformity against GC-content bias resulted from different DNA fragmentation and library preparation kits were compared by plotting normalized coverage for a wide GC-content (8% - 88%).
sparQ DNA Frag & Library Prep Kit is tunable to the desired fragment size. 100 ng Human gDNA was subjected to fragmentation with a series of incubation time points (3 – 18 min). After fragmentation, DNA samples were purified and then visualized using Agilent High Sensitivity DNA Kit.
Simply select the desired fragment size and input DNA amount. If input DNA falls between values displayed on the graph, an estimate can be used for optimizing fragmentation times.