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Dithiothreitol (DTT, Cleland's reagent) ≥99.4% for biotechnology

Supplier: VWR Chemicals

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Synonyms: DL-1,4-Dithiothreitol , DTT , cis-2,3-Dihydroxy-1,4-butanedithiol , (R*,R*)-1,4-Dimercapto-2,3-butandiol

0281-25GEA 12989 INR InStock
0281-100G 0281-25G 0281-1KG 0281-5G 0281-2500
Dithiothreitol (DTT, Cleland's reagent) ≥99.4% for biotechnology
Dithiothreitol (DTT, Cleland's reagent)
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.

DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.

It is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).


Formula: C₄H₁₀O₂S₂
MW: 154,25 g/mol
Boiling Pt: < 125 °C (1013 hPa)
Melting Pt: 42…44 °C
Flash Pt: >110 °C (open cup)
Storage Temperature: Refrigerator
 MDL Number: MFCD00004877
CAS Number: 3483-12-3
EINECS: 222-468-7
UN: 0000
https://b2b-in-gqp.vwr.com/stibo/web/std.lang.all/98/19/2299819.jpg
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Specification Test Results
Abs.@283 nm (0.02 M, Water) ≤0.05
DNase none detected
Loss on Drying <0.5%
Melting Point 39 – 43 °C
Oxidised DTT <0.5%
Protease none detected
Purity (-SH Content) >99.4%
RNase none detected
Solubility (5%, Water) pass

 

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