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Pierce™, Melon™ IgG spin Purification Kits, Gel

Supplier: Thermo Fisher Scientific

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Melon™
PIER45206EA 0 INR InStock
PIER45206
Pierce™, Melon™ IgG spin Purification Kits, Gel
Protein Purification Protein Purification Kits
The Pierce™ Melon™ Gel IgG Spin Purification Kit is a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

  • Higher recovery and purity
  • Effective for subclasses of IgG that bind poorly to Protein A or G
  • Regenerable, support can be regenerated and used for up to six purifications
  • Compatible, amine free buffer does not require removal or neutralisation

The protocol does not require any desalting or extreme pH conditions, enabling antibodies to retain their biological properties. Melon™ gel consistently offers better than a 90% recovery rate with better than 80% for all antibodies, including some subclasses that are generally unattainable using Protein A or G purification methods.

Sufficient for IgG purification from approx. 25×0,1 ml serum samples (total approx. 25 mg IgG from serum).

These kits enable purification of antibodies from serum and other sample types. Melon Gel Resin binds and removes serum proteins, thereby allowing pure IgG to be collected in the flow-through fraction. The procedure is gentle because no harsh elution conditions are involved.
Melon Gel Resin contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because Melon Gel Resin is not a bind-and-release support, it is extremely fast and gentle to antibodies, resulting in antibody preparations of high purity and high activity.

Melon Gel Resin works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with Melon Gel Resin are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralise or desalt the recovered antibody to remove harsh elution buffers.

Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.

Delivery information: Kit includes: Melon™ Gel support (3 ml); Melon™ Gel purification buffer (dry mix to make 100X), (100 ml); spin columns (0,8 ml, 27 columns); microcentrifuge tubes (2 ml, 30 tubes).

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