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HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid), powder acid-free, Corning®

Supplier: MEDIATECH

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61-034-RR 61-034-RO 61-034-RM
MDTC61-034-RREA 22766.58 INR InStock
MDTC61-034-RR MDTC61-034-RO MDTC61-034-RM
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid), powder acid-free, Corning®
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)
HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.


Formula: C₈H₁₈N₂O₄S
MW: 238.31 g/mol
Storage Temperature: Ambient
 MDL Number: MFCD00006158
CAS Number: 7365-45-9
Merck Index: 13,04672
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