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qPCR SuperMixes, PerfeCTa®

Supplier: QIAGEN BEVERLY

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qPCR SuperMixes, PerfeCTa®
Nucleic Acid Reagents qPCR/RT-qPCR Enzymes and Kits
PerfeCTa® qPCR SuperMixes are 2X concentrated, ready to use reaction cocktails containing all components except primers, probe and template for qPCR.

  • Broad dynamic range for greater reliability
  • Superior antibody mediated hot start enables higher specificity leading to more accurate quantification

These SuperMixes include buffers and stabilisers optimised for maximum PCR efficiency, reproducibility and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. The enhanced specificity of PerfeCTa® qPCR SuperMixes suppresses cross reactivity between homologous sequences, improving detection and discrimination in SNP applications. A key component is AccuStart™ Taq DNA polymerase that contains monoclonal antibodies, which bind and inactivate the polymerase prior to the initial PCR denaturation step. Upon heat inactivation (2 minutes at 95 °C) the antibodies irreversibly denature releasing fully active, unmodified Taq DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. Perfecta® qPCR SuperMixes are formulated with and without reference dye to provide compatibility with specific Real-Time qPCR instruments.

Sample kits are available, please enquire.


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