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PerfeCTa® qPCR ToughMix®

Supplier: Quantabio

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PerfeCTa® qPCR ToughMix®
733-2244EA 394250 INR InStock
733-2244 733-2243 733-2098 733-2252 733-2251 733-2097 733-2096 733-2248 733-2247 733-2094 733-2093 733-2092 733-2091 733-2090
PerfeCTa® qPCR ToughMix®
Nucleic Acid Reagents qPCR/RT-qPCR Enzymes and Kits
PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of PerfeCTa® qPCR ToughMix® is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeCTa® qPCR ToughMix® delivers exceptional performance with either fast or conventional PCR cycling protocols.

Delivery information: Kits are available with or without ROX reference dye. All kits include 2X reaction buffer containing optimised concentrations of MgCl₂, dNTPs (dATP, dCTP, dGTP, dTTP), hot start DNA polymerase, AccuVue™ blue qPCR dye, and stabilisers. UNG versions include uracil DNA glycosylase.

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