Hot start is a well established and convenient method to improve specificity of PCR. However, conventional technologies, like antibody mediated inhibition or chemical blocking of DNA polymerases, carry a few limitations - long initial activation steps may be required, the performance of the DNA polymerase can be reduced or specificity control may be compromised. HotMaster Taq DNA Polymerase uses an innovative technology to achieve hot start PCR. A thermostable inhibitor of the Taq DNA polymerase is released at high temperatures, thereby immediately activating the enzyme. The HotMaster technology not only provides hot start control at reaction set-up, but also cold stop during the annealing step of each cycle of PCR.
- Hot start/cold stop technology requires no enzyme activation step making it ideal for fast PCR protocols
- Convenient room temperature reaction set-up
- Highly specific amplification
- Minimal optimisation with self adjusting Mg2+ buffer technology
Product comprises: HotMaster Taq DNA Polymerase (5 U/µl) and 10X HotMaster Taq Buffer with 25 mM Mg2+.
