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HotMasterMix (2,5X)

Supplier: Quantabio

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733-2474EA 91250 INR InStock
QUNT2200410
HotMasterMix (2,5X)
Nucleic Acid Reagents End-point PCR Enzymes and Kits
HotMasterMix is a ready to use 2,5X reagent mix for performing high throughput PCR (based on hot start/cold stop technology). The HotMasterMix guarantees the highest specificity and consistency. The key component of the mix is the HotMaster Taq DNA polymerase that consists of a pre-mixed HotMaster inhibitor that binds and blocks the substrate-binding site of HotMaster Taq in a temperature-dependent manner. At temperatures above 60 °C, the inhibitor is released and the HotMaster Taq becomes activated (hot start). At temperatures below 60 °C, the inhibitor inactivates the Taq polymerase (cold stop) preventing non specific primer annealing or primer-dimer formation. Unlike many conventional hot start PCR techniques, such binding-releasing equilibrium is reversible throughout the PCR cycle.

  • Highly specific PCR
  • Ready to use master mix format
  • Convenient storage at 4 °C eliminates freeze and thaw cycles
  • Minimal optimisation with self adjusting Mg²⁺ buffer technology

Product comprises: Taq DNA polymerase (1,0 U/50 µl reaction), KCl, Mg²⁺ and dNTPs.

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