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Supplier: OMEGA BIOTEK
Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

Supplier: OMEGA BIOTEK
Description: The E.Z.N.A.® miRNA isolation kit uses a rapid procedure to isolate small RNAs, such as micro RNA (miRNA), small interfering RNA (siRNA), and small nuclear RNA (snRNA), from wide range of tissues and cells. The fast and efficient silica membrane based method isolates total RNA ranging in size from kilo based down to decamers. The kit also provides reagents and a procedure to enrich the population of RNAs that are 200 bases and smaller, which enhances the sensitivity of small RNA detection by solution hybridisation, Northern analysis, and other methods.
Supplier: OMEGA BIOTEK
Description: This second generation plasmid miniprep kit yields 40 to 70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 ml to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.
Supplier: OMEGA BIOTEK
Description: E-Z® 96 DNA plates are silica glass fibre plates can bind up to 50 μg of genomic DNA or 20 μg of plasmid DNA per prep. Each well has a capacity of 1 ml and the plates are semi skirted. E-Z 96 DNA plates can be processed in vacuum manifolds and/or by centrifugation.

Catalog Number: (A0965.9010)
Supplier: APPLICHEM LIFESCIENCE
Description: Powder mixture to prepare PBS buffer (10X) according to Dulbecco.
UOM: 1 * 1 KIT


Supplier: HICHROM SELF MANUFACTURED
Description: These ultra-inert Avantor® ACE® Excel® C8 columns provide great reproducibility and column lifetime. These stainless steel columns are available in a wide range of particle sizes and dimensions from capillary to preparative.

Catalog Number: (0966-400ML)
Supplier: VWR Chemicals
Description: Phenol / Chloroform #2- A 25:24:1 mixture of phenol, chloroform, and isoamyl alcohol useful for preparing RNA with maximal recovery of poly (A+) RNA for subsequent mRNA purification and generation of cDNA.
UOM: 1 * 400 mL

MSDS


Supplier: HICHROM SELF MANUFACTURED
Description: Avantor® ACE® Excel® C18-Amide is a uniquely designed stationary phase offering improved retention of polar compounds, enhanced phenolic resolution and alternative selectivity to C18 bonded columns. These stainless steel columns are available in a wide range of particle sizes and dimensions from capillary to preparative.

Supplier: HICHROM SELF MANUFACTURED
Description: Avantor® ACE® AQ columns provide great reproducibility and column lifetime. These stainless steel columns are available in a wide range of particle sizes and dimensions from capillary to preparative.

Supplier: VWR Chemicals
Description: TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).
Catalog Number: (84864.600)
Supplier: VWR Chemicals
Description: Dose for preparation of 1 litre of solution of concentration 0.1 mol/l
UOM: 1 * 170 mL

Supplier: HICHROM
Description: Avantor® Partisil® was one of the first commercially available irregular silicas with a large surface area giving it a high loading capacity for preparative work.

Supplier: VWR Chemicals
Description: Solutions prepared from AnalaR® NORMAPUR® grade analytical reagents.
Supplier: OMEGA BIOTEK
Description: The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

Supplier: HICHROM SELF MANUFACTURED
Description: Avantor® ACE® SuperC18 columns provide high stability across an extended pH range. These stainless steel columns are ideal for use with LC/MS compatible buffers and can be used with both MeOH and MeCN mobile phases.

Supplier: VWR Chemicals
Description: Lysogeny broth (LB) is a nutritionally rich medium used for the propagation and maintenance of <i>E. coli. </i>It is also widely used for the preparation of plasmid DNA and recombinant proteins.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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