How to Choose a Chromatograhy Column

Webinar

Thursday, 14th April, 2022  
1:30PM to 03:00PM IST

Register

There have been significant advances in column technology since the early day of chromatography. These advances have seen a greater understanding of the dispersion processes that occurs within the column and how to reduce them, resulting in sharper chromatographic peaks. However, chromatography is not just about sharp peaks, it is about separating two or more components and so a critical parameter is the column selectivity. This presentation will focus on why column selectivity is so important, initially reviewing the resolution equation to validate the notion that it is selectivity that drives a separation. The presentation will then move on to discuss how to effectively characterise the array of columns that are available for separation scientists, and how this information can be utilised to guide column selection and ultimately, improve the performance of the separation.

Separation scientists should have a tool-box of tricks that they can employ to optimise the separation of a critical pair. An important aspect of this is a suite of columns that can drive selectivity differences. In this part of the presentation a set of method development columns will be presented that will highlight how column selection can be critical and what types of columns should be employed to allow fast optimisation of the chromatographic method. Six different column chemistries will be investigated and the impact that these have on the selectivity of a series of test compounds will be highlighted.

For separations to be optimal a series of other parameters also need to be assessed/optimised and the final part of the presentation will discuss how the column selection can be integrated with the other parameters such as mobile phase composition, pH and temperature to optimise the separation process. A considered design of experiments approach will be taken to allow full optimisation of the separation process.

For more information, please contact us at webinar@avantorsciences.com

Presented By:

   

Tony Edge
 

Tony Edge is the Site Director for the Avantor Centre of Excellence for Chromatography in the UK, heading a team of specialist scientists in developing next generation stationary phases for HPLC. He has worked in both manufacturing and also industry, having periods of employment at LGC and also AstraZeneca as well as ThermoFisher Scientific and latterly Agilent Technologies. In 2008, he was fortunate enough to be awarded the Desty memorial lecture for his contributions to innovating separation science, and in the same year also won a clinical excellence award from AstraZeneca. Tony’s current interests are centred on improving the extraction process and high-temperature chromatography. Tony was awarded an honorary fellowship at the Liverpool university, where he lectures on separation science, and also lectures at Keele University on Management in Analytical Science. Tony is also the President of The Chromatographic Society in the UK and a contributing editor for the Chromatography Today magazine. Tony is also part of the Reid Bioanalytical conference organising committee, and a permanent member of the scientific committee for the International Symposium on Chromatography.