You Searched For: Vectors,+Plasmids+and+Libraries

Supplier: MIRUS

Description: Fluorescently labelled plasmids ideal for <i>in vitro</i> and <i>in vivo</i> tracking.

Supplier: VWR Chemicals

Description: Ribozol™ RNA Extraction Reagent is a single phase phenol solution optimized for the isolation of total RNA from a variety of cell and tissue types.

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Supplier: QIAGEN BEVERLY

Description: The sparQ DNA Frag and Library Prep kit optimises the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimising DNA over fragmentation while greatly simplifying library.

Supplier: QIAGEN BEVERLY

Description: An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.

Catalog Number: (N788-KIT)

Supplier: VWR Chemicals

Description: The phenol-free total RNA purification kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses.

UOM: 1 * 1 KIT

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Supplier: OMEGA BIOTEK

Description: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

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Catalog Number: (N643-KIT)

Supplier: VWR Chemicals

Description: RiboZol™ Plus RNA Purification Kit purifies and recovers all sizes and species of RNA, including large RNAs such as ribosomal RNA (rRNA) or messenger RNA (mRNA), and small RNAs <200 nucleotides, including microRNAs (miRNA) and small inhibitory RNAs (siRNA). The kit combines the benefits of organic RNA extraction using RiboZol™ RNA extraction reagent with the ease and convenience of spin column technology. Firstly, RiboZol™ RNA extraction reagent extracts total RNA from cells or tissue, including difficult sample types. Subsequent column chromatography employs a proprietary resin as the separation matrix to yield up to 35 μg of highly purified total RNA.

UOM: 1 * 1 KIT

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Supplier: OMEGA BIOTEK

Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

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Supplier: OMEGA BIOTEK

Description: E.Z.N.A.® Fastfilter® Plasmid kits rapidly purify plasmid DNA utilising lysate clearance filter syringes, whilst E-Z 96® Fastfilter Plasmid kits employ lysate clearance plates in a 96-well format. Fastfilter® kits allow midi- and maxi-scale plasmid isolation in less than 40 m.

Supplier: OMEGA BIOTEK

Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, <i>E.coli</i> strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription<i> invitro</i>, and other applications.

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Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.® Plasmid Mini Kit I is available in either V-spin or Q-spin column format. V-spin column formats have an attached lid while Q-spin columns do not. Both versions can be used for centrifugation or vacuum protocols.

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Supplier: VWR Chemicals

Description: Lysogeny broth (LB) is a nutritionally rich medium used for the propagation and maintenance of <i>E. coli. </i>It is also widely used for the preparation of plasmid DNA and recombinant proteins.

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Supplier: VWR Chemicals

Description: Polyethylene glycol is used for the isolation of plasmid DNA and the precipitation of phage.

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Catalog Number: (733-2895)

Supplier: QIAGEN BEVERLY

Description: sparQ Universal Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms.

UOM: 1 * 100 Assays

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Supplier: OMEGA BIOTEK

Description: Isolate total RNA from cells or soft tissues using spin column.

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