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Washing+Buffers
Catalog Number:
(0883-100ML)
Supplier:
VWR Chemicals
Description:
Phenol:Chloroform pH 6.7/8.0 is premixed with isoamyl alcohol (25:24:1). A phenol:chloroform mixture is ideal for the extraction of protein from DNA preparations. Packaged at pH 6.7, this product is accompanied by a separate alkaline buffer which allows the researcher to increase the pH to 8.0. Doubly distilled, high purity phenol packaged under nitrogen. Safer, easier alternative to crystalline phenol.
UOM:
1 * 100 mL
Catalog Number:
(700-0292)
Supplier:
VWR Collection
Description:
The shiroGEL mini vertical PAGE system is modular, allowing PAGE and electroblotting to be carried out in the same tank, simply by changing the inserts. The system features inserts for each application, and a common buffer tank in which to run them. Each insert has its own electrode assembly, which connects to the lid of the buffer tank. For safety, the power leads connect directly to the lid. When the lid is removed, power is disconnected from the system. The inserts also have a small pad that fits into position in the lid. This ensures that the lid is always properly placed and aids in its removal. The buffer tank is moulded to prevent separation and leaking. A variety of spacers, combs and other accessories are available to customise the system. All spacers and combs are colour coded or clearly labelled to indicate thickness.
UOM:
1 * 1 items
Supplier:
VWR Collection
Description:
The shiroGEL vertical 20 offers application flexibility and is distinguished by its ease of gel casting and set-up, requiring only four screws to secure up to four 20×20 cm gels. This vertical screw clamp technology distributes pressure evenly along the height of gel rather than in the centre to eliminate plate bowing and gel compression, while maintaining a leakproof seal during casting. A built-in inner buffer chamber within the PAGE insert allows set up to be completed without inclusion of an upper buffer chamber. These systems are ideal for 1D and 2D SDS-PAGE, native, preparative, gradient and high resolution nucleic acid electrophoresis.
Catalog Number:
(M111-100ML)
Supplier:
VWR Chemicals
Description:
Strong detergent utilised in lysis buffers for the complete disruption of membranes and denaturation of proteins.
UOM:
1 * 100 mL
Supplier:
RETSCH
Description:
The ZM 200 is a powerful ultra-centrifugal mill for both demanding grinding tasks and efficient routine work. ZM 200 reduces the size of soft to medium-hard and fibrous materials. Ideal for rapid yet gentle preparation of analytical samples of materials such as chemicals, drugs, spices, coal, synthetic resins, plastics, pharmaceutical raw materials and finished products, plant constituents and surfactant determination in washing powders. For grinding larger amounts the use of a paper filter bag or a cyclone with 3 or 5 litre collecting receptacle is recommended. The sample is then cooled by the airstream and discharged more rapidly from the grinding chamber via the passage receptacle.
Catalog Number:
(N806-2X1.25ML)
Supplier:
VWR Chemicals
Description:
Ready PCR Mix, 2X offers a single-step procedure for performing PCR reactions followed by analysis on agarose gels without the addition of loading buffer. All components for assembly and performance of PCR reactions as well as loading and visualisation of PCR products on agarose gels are included. The user supplies primer and template DNA.
UOM:
1 * 1 KIT
Supplier:
Avantor
Description:
Buffer for liquid chromatography and other molecular biology applications. Lot analysis on label.
Supplier:
VWR Chemicals
Description:
These DNA loading dyes are used to load DNA samples to agarose or SDS DNA gels for gel electrophoresis.
Supplier:
VWR Collection
Description:
PerfectBlue S and M tank electroblotters are designed to rapidly transfer nucleic acid or protein fragments from up to four polyacrylamide gels at a time to nitrocellulose, nylon or PVDF membranes. The colour coded cassettes allow for easy assembly of transfer sandwich and error-free transfer. The large buffer capacity and integral cooling allow extended transfer times and high voltage/high current operation for the transfer of high molecular weight proteins.
Supplier:
VWR Chemicals
Description:
VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.
Supplier:
VWR Chemicals
Description:
A biological buffer used for cell culture, <i>in vitro</i>, enzyme assays and some electrophoretic applications at physiological pH.
Supplier:
VWR Chemicals
Description:
Reagent for stabilisation of buffer formulations and electrophoresis of biological molecules.
Supplier:
VWR Collection
Description:
For 16×14 cm gels, the Twin M is a versatile system for SDS PAGE applications, in addition to non denaturing electrophoresis of proteins, SSCPs and dinucleotide repeats. With the enlarged upper buffer chamber, water circulation system and heat-sinking aluminium ceramic plates, the Twin M provides high speed separation while maintaining straight, sharp bands.
Supplier:
BIOWEST
Description:
In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.
Supplier:
VWR Collection
Description:
Combined electrodes providing fast, accurate and stable pH measurements, ideal for control quality and research and development use.
Catalog Number:
(662-2351)
Supplier:
VWR Collection
Description:
These economical, robust, and waterproof pH meters are supplied in complete, ready to use kits that incorporate a robust carrying case, electrode and temperature sensor with different cable lengths and buffers.
UOM:
1 * 1 items
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the
 is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the
is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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