Environmental DNA screening: Accelerate 16S workflows in inhibitor rich samplesWebinarReplayTaxonomic identification of bacterial species is vital in many environmental and healthcare applications to inform on the appropriate course of action. The 16S rRNA gene amplicon sequencing workflow provides a low cost, efficient method to identify the phylogenetic composition of a mixed sample of bacteria. 16S library preparation typically takes 3 hours and it involves two rounds of PCR and two bead clean up steps. We present an exciting, renewed 16S library preparation protocol using Quantabio’s repliQa HiFi ToughMix to reduce overall costs while accelerating time to results. Presented by: Ishna is the Quantabio field application specialist for EMEA. She completed her PhD in Chemical Biology at the University of Southampton, UK then took a post-doctoral position in the department of oncology at the University of Oxford, UK. Since, she has worked in the biotechnology field, developing NGS library preparation reagents and instruments and has extensive, hands-on experience with a wide range of NGS applications. As part of the field application team, she is using this expertise to support customers with NGS applications and develop new, optimised protocols with the sparQ library preparation reagents. |
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